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HDL-Cholesterol Kit
Cholestest®N HDL

*Read carefully the package insert before use.

INDEX

Cholestest®NHDL is a liquid reagent for the measurement of HDL-cholesterol by direct method, using differences in specificities of detergent to lipoprotein. Because the reagent doesn't include polyanions or magnesium salts, it allows accurate measurement of samples with rich globulin or rich TG. The reagent forms no insoluble precipitates upon addition of an alkaline washing solution, so it will impose no loads on instrument.

Features

  • Includes no flocculants or divalent metal ions
  • Allow accurate measurement of samples with rich globulin or rich triglyceride
  • Free of the influence of bilirubin
  • Doesn't contaminate instrument

Measurement Principle

Reaction 1
The reaction accelerator allows free cholesterol on the surface of LDL or VLDL to react with the enzyme.
H2O2 produced in the reaction is eliminated by peroxidase, thus no color dye occurs.
Reaction 2
The special detergent causes only HDL to be solved to allow reaction of HDL-cholesterol and the enzyme.
H2O2 produced in the reaction condenses 4-amino antipyrine and DSBmT to produce color compound in the presence of peroxidase.
Name Package Shelf-life Storage
Cholestest®N HDL Enzyme solution (1) 2×60mL 24 months 2-10°C
Enzyme solution (2) 2×20mL
Cholestest®N Calibrator* 3×2.0mL 12 months

*Cholestest®N Calibrator is not an in vitro diagnostic regent.

Reference Data
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We prepared this page for medical staff (doctors, dentists, pharmacists, clinical laboratory technicians, nurses, etc.) in Japan to ensure the correct use of our products. You should be aware that the information provided is not intended for overseas medical staff and the general public.