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LDL-Cholesterol Kit
Cholestest®LDL

*Read carefully the package insert before use.

INDEX

Cholestest®LDL is a liquid reagent for the measurement of LDL-cholesterol concentrations using differences in affinity between lipoproteins and detergents. The reagent requires no precipitation procedures and can be used on automated analyzers. It also shows a high correlation with the ultracentrifugal method.

Features

  • Intended for the direct method, which requires no complex operations
  • Shows a high correlation with the ultracentrifugal method
  • Is a liquid reagent that can be used on various types of automated analyzers
  • Free of the influence of lipemia (up to 1500 mg/dl of triglyceride)

Measurement Principle

Enzymatic measurement

Reaction 1
Detergent 1 doesn't change the structure of LDL, but changes instead the structure of chylomicron (CM), VLDL, and HDL, which triggers the enzymatic reaction of cholesterols in these lipoproteins with cholesterol esterase (CE) and cholesterol oxidase (COD). Hydrogen peroxide (H2O2) formed in the reaction is eliminated by the actions of peroxidase (POD) and 4-aminoantipirine (4-AA).
Reaction 2
Detergent 2 changes the structure of LDL, which remains intact in Reaction 1, to trigger an enzymatic reaction. Our reagent includes a coloring agent N,N-bis(4-sulfobutyl)-m- toluidine disodium (DSBmT), which reacts with hydrogen peroxide produced from LDL- cholesterol to exhibit a violet-blue color. This reaction is used for determination by colorimetry.
Name Package Shelf-life Storage
Cholestest®LDL Enzyme solution (1) 2×60mL 24 months 2-10°C
Coloring solution (2) 2×20mL 24 months
Cholestest®N Calibrator* 3×2.0mL 12 months

*Cholestest®N Calibrator is not an in vitro diagnostic reagent.

Reference Data (1)
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We prepared this page for medical staff (doctors, dentists, pharmacists, clinical laboratory technicians, nurses, etc.) in Japan to ensure the correct use of our products. You should be aware that the information provided is not intended for overseas medical staff and the general public.